五分时时彩计划

您好,欢迎光临欣博盛生物科技商城!
全国服务热线:4006-800-892
关注我们
  • 会员中心
  • 会员中心
    询价列表
    0

    最新加入的商品

    0 件商品 合计:¥ 0
  • 会员中心
    购物车

    最新加入的商品

    件商品 合计:¥ 0

全部产品分类

自主品牌
当前位置:五分时时彩 > 新闻资讯 > 新品发布 > InvivoGen 新品推荐:核酸转染增强试剂——NATE™

InvivoGen 新品推荐:核酸转染增强试剂——NATE™

时间:2019-05-10 作者:市场部 文章来源: 浏览:350

InvivoGen 新品推荐:核酸转染增强试剂——NATE™


产品介绍:

NATE™是InvivoGen设计的一种核酸转染增强剂可以提高难转染细胞的瞬转与稳转效率,尤其适合人的单核细胞与小鼠巨噬细胞等难转染的细胞,如外周血单核细胞(THP-1)和小鼠单核巨噬细胞白血病细胞(RAW 264.7),且只需在平时加转染试剂操作前30min加入NATE™即可。值得注意的是,NATE对细胞温和,不会对细胞培养产生任何进一步的毒性。

 

在真核细胞转染过程中,外源性核酸(如质粒)的主要障碍会被胞质传感器检测,cGAS/STING、AIM2炎性小体和LC3介导的自噬。这些防御信号级联的激活常常会降低转染率和细胞存活率,特别是在难以转染的细胞(如免疫细胞)中会更明显。当使用NATE™时,这些核酸传感通路将被抑制,从而在转染过程中保护质粒并促进其表达。



产品特色:

● 与常用转染试剂 (e.g. GeneXPlus, Lipofectamine® LTX, and jetPRIME®) 及物理方法兼容。

● 更高的转染率,也适用于大质粒 (> 10kb)。

● 在所有的转染测试方案中都显示对细胞温和,没有毒性。


产品信息:

Product Name

Unit Size

Cat. code

NATE™

1 mL (100 reactions)

lyec-nate


应用举例:

Top - Transient transfection of an ~3 kb GFP-expressing plasmid into THP-1 cells was performed using GeneXPlus both in the absence (left) and presence (right) of NATE™. After 48 hours, the transfected cells expressing GFP were visualized by fluorescence microscopy. Bottom - Transient transfections of an ~3 kb GFP-expressing plasmid (left) and an 8 kb GFP-expressing plasmid (right) into THP-1 cells was performed using commonly used transfection methods such as GeneXPlus, Lipofectamine® LTX, jetPRIME®, and nucleofection。 This was performed both in the presence (green) and absence (yellow) of NATE™。 After 48 hours, transfection efficiency (% GFP-expressing cells) was measured using flow cytometry。 Data are presented as a fold change compared to transfection without NATE™ 


Top - Transient transfection of an ~3 kb GFP-expressing plasmid into RAW 264。7 cells was performed using Lipofectamine® LTX both in the absence (left) and presence (right) of NATE™。 After 48 hours, the transfected cells expressing GFP were visualized by fluorescence microscopy。 Bottom - Transient transfection of an ~3 kb GFP-expressing plasmid (left) and an 8 kb GFP-expressing plasmid (right) into RAW 264.7 cells was performed using commonly used transfection methods including Lipofectamine® LTX, jetPRIME®, FuGENE®, and nucleofection. This was performed both in the presence (green) and absence (yellow) of NATE™. After 48 hours, transfection efficiency (% GFP-expressing cells) was measured using flow cytometry. Data are presented as fold change compared to transfection without NATE™.


Stable transfection of an ~10 kb SEAP‑expressing plasmid into RAW 264.7 cells was performed using Lipofectamine® LTX both in the absence (left) and presence (right) of NATE™. After 10 days in selection with Blasticidin, the number of stable clones expressing SEAP (blue wells) was visualized using QUANTI‑Blue™, a SEAP detection reagent.



详情请咨询 invivogen 中国授权代理-欣博盛生物科技

全国服务热线: 4006-800-892       邮箱: market@neobioscience。com

深圳: 0755-26755892         北京: 010-88594029         上海: 021-34613729          

广州:18024516375          香港: 852-69410778

代理品牌网站: kounte.com

自主品牌网站: www.neobioscience.net

糖型分析网站: 

五分时时彩技巧 五分时时彩技巧 三分时时彩计划稳赢版 五分时时彩怎么玩 五分时时彩官方 五分时时彩 五分时时彩下载 三分时时彩计划 三分时时彩计划稳赢版 五分时时彩官网